Syed Muzammil Afzal
Sri Shivani College of Pharmacy, India
Title: Development & comparison of docetaxel lipid nanoemulsions by different strategies for tumor targeting
Biography
Biography: Syed Muzammil Afzal
Abstract
The aim was to develop tumour-targeted docetaxel lipid nanoemulsions with four strategies for improving the antitumor activity and compare their targeting efficiencies. The O/W lipid nanoemulsions (L), were prepared by homogenization followed by ultrasonication process. The size of globules and zeta potential were measured by Malvern Zetasizer. The drug content and entrapment efficiencies for the lines were determined by HPLC. The in-vitro cytotoxic studies of the delivery systems were performed on MCF-7 and HeLa cells. The IC50 values of targeted lines on both the cell lines were statistically significant (P<0.05) when compared to DS (Docetaxel solution) & DLNE (Docetaxel LNE). The in-vivo antitumor activity was tested on solid tumours induced in C57BL/6 mice. This study revealed that the percentage a tumour inhibition, when compared with the control (untreated), were found to be 55.62%, 70.80%, 89.31%, 54.25%, 80.0% and 84.66% for the DLNE, PLNE, FLNE,SALNE, ALNE and TFLNE formulations respectively. Further, in-vivo distribution studies were carried out in breast cancer, MDA-MB231 induced tumours in Balb/c mice. The LNEs were loaded with fluorescent DiD oil and the distribution in different organs after 6hrs was tracked by small animal in-vivo imaging system. When compared with the DLNE the average radiance values at the tumour site for the formulations PLNE, FLNE, SALNE, TFLNE and ALNE were found to be increased by 2.31, 4.81, 1.35, 3.54 and 3.04 folds respectively. The tumour targeting efficiency was found to be in the order FLNE> TFLNE>ALNE> PLNE>SALNE>DLNE by an imaging study.