12^th Annual Pharma Middle East Congress Sep 25-26, 2017 Dubai, UAE

Therapeutic products 1- Research and Development for production of high efficacy recombinant Human Growth Hormone. (rhGH) is approved for the treatment of multiple human diseases resulting from hGH deficiency. rHGH has been produced in a system for production of recombinant proteins using E. coli and pTXB1 expression system, a single band at 22 kDa. The purified protein identity was verified using mass spectrometry. 2- Construction of plasmid vector for protein expression in prokaryotic cells Named as (pTBRI), Innovative plasmid allows the user to cloning and expression of genes to be the first step in the production of therapeutic proteins. 3- Research and Development for production of high efficacy recombinant human IFN-α2b conjugated with multiply branched high molecular weight PEG polymer for treatment of HCV infected patients. The developed production process included feed batch fermentation, purification, solubilization and refolding of protein to its native 3ry structure and purification followed by Pegylation of the purified protein and purification of monopegylated IFN-α2b. 4-New formulation of praziquantel using nanotechnology to achieve greater availability and higher efficiency against schistosomiasis. Health tools 1- A new design of intubating laryngeal mask airway (I-LMA) in cases of difficult intubation. It contains a bundle of tubes with multiple holes in their walls to permit gas flow freely. (C) 2- A device for anesthesia using distilled-water injector to get rid of carbon dioxide, physically to be added as an alternative to the caustic soda lime used in regular anesthesia devices. (D) 3- Manufacturing of vertical-flow strip nano-chip kit with graphine nanoparticles and evaluating its role as a simple, rapid, low-cost, sensitive, specific, and user-friendly diagnostic tool for diagnosis of multiple parasitic diseases (e.g. schistosomiasis, fascioliasis, filariasis and malaria) in one kit. (E) 4-A program for the diagnosis of parasites through the Internet based definition and diagnosis of parasites using a different website supported all forms and types of parasites diagnosis. It assists all medical, scientific and educational frameworks in the diagnosis of parasites.

Background: Self-efficacy might affect the outcome of the treatment by interfering with patients’ behavior. Successful self-management of any disease is a role of self- efficacy, belief or the feeling of confidence in one’s self-management abilities. Successful daily self-management of diabetes by predictors glycemic control is the ultimate goal to the achievement of diabetic control outcomes. Objectives: The aim of this study was to determine the influence of selfcare management behaviors and self-care efficacy on achieving the glycemic control, specifically glycosylated hemoglobin levels (HA1C). Methodology: This was a cross- sectional study conducted at the Diabetes Clinic; King Faisal Hospital; Taif city; Kingdom of Saudi Arabia during the period of June –August 2016. The study was approved by the Research and Ethical Committee, King Faisal Hospital, Taif, KSA. study included patients aged > 20 years and diagnosed with Type 2 diabetes mellitus (T2DM) at least one year before commencement of data collection. The data was collected by interviewing the patients using faceto- face interview method, while clinical data was extracted from medical records. A pre-tested and validated questionnaire composed of four sections was used for the purpose of data collection. The first part was designed to collect data on patients' s sociodemographic and clinical characteristics. The second part was designed to collect data on diabetes management self-efficacy using the Diabetes Management Self-Efficacy Scale (DMSES). Data was processed using the software (SPSS). Result: A total of (n=300) was included, 49.3% male and 50.7%female. Off all, 132 (44%) of patients were classified as having high self-efficacy across all domains. Predictors of high level of self- efficacy were living in the town (P = 0.006) and being married (P= 0.003). There were significant associations between self-efficacy by domain and adherence to the specific self-care activity corresponding to that domain. Patients found to have high level of self-efficacy in the management nutrition, physical exercise and weight control, and medical treatment were 154 (51.3%), 112 (37.3%) and 270 (90%) respectively. Conclusion: Living in the town and being married were found to be the significant predictors of high self-efficacy. The patients who achieved therapeutic goal of HA1C<7% were 84(28 %) and the control of the disease was significantly better among females compared to males. There were many gaps related to management self-efficacy of diabetes and adherence to self-care activities. Educational interventions to improve the situation are urgently needed.

Introduction: Seeking new antimicrobial agents to overcome the emergence of antibiotic resistance, some novel synthetic chalcone derivatives were evaluated for their antibacterial activity. The study targeted chalcone derivatives due to the great antimicrobial activity shown by compounds from this family in other previous studies. Methodology: Four synthetic chalcone derivatives TChD-(01-04) were synthesized according to Claisen−Schmidt condensation and their structures were deduced using various spectral techniques (IR, MS, 1H-NMR, and 13C-NMR). These compounds were evaluated for their antibacterial activity against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) using microdilution assay in comparison with DMSO (as vehicle control) and ciprofloxacin and gentamicin in term of MIC, Emax and EC50 values. Also the pharmacodynamic interactions between the synthesized chalcone derivatives were determined by using Checkerboard assay, and the antibacterial activity of each combination was compared with those of each drug alone and with ciprofloxacin and gentamicin. Results: MIC values of the synthesized TChDs ranged between 256 - >512 μg/mL against S. aureus with Emax between 60.94% ± 5.58 to 99.39% ± 0.61, and MIC ≥512 μg/mL against E.coli Emax range 84.58% ± 5.19 to approximately 100%, Among the tested compounds TChD-03 was the best with high efficacy against both strains and the best MIC value against S. aureus. On the other hand the FIC index of TChD-02 with TChD-03 combination showed additive effect against both S. aureus and E. coli. Conclusion: The synthesized chalcone derivatives showed promising antibacterial activity, which may be enhanced by improving their physicochemical properties.

Hosniyeh Amini has completed her masters at the age of 27 years from Tehran. She studied about effect of drugs in cancer and mental.

Breast cancer is a very important epidemiologic problem and the most common cause of death in women. Oxidative stress play an important role in this disease. Antioxidants such as A, C, E, K, D vitamins have drawn the attention of many scientists for reducing oxidative stress. AquADEKs is one of the antioxidant supplements. The purpose of this study was to investigate the effect this supplement in reducing oxidative stress in breast cancer. In this applied research, 38 BALB-C mice were divided into 4 groups. In the sera of the mice, the levels of catalase, superoxide dismutase, and total antioxidant capacity were measured by the Elisa method. Data analysis was performed using ANOVA and Graphpad. The catalase level significantly increased in mice that received vitamin supplements and were implanted with cancer cells (P = 0.049) this relationship was not significant with vitamin D. Superoxide dismutase levels significantly increased in the mice that received vitamin supplements and were implanted with cancer cells (P = 0.024) but this relationship was not significant with vitamin D. The total antioxidant capacity increased significantly in mice that received vitamin supplements and were implanted with breast cancer cells (P < 0.001) and this relationship was significant with vitamin D. The use of AquADEKs as supplement had a significant correlation with the levels of catalase, superoxide dismutase, and total antioxidant capacity. Antioxidant markers can be improved with the use of AquADEKs if this supplement is imported and available in market.

Heyam Saad Ali has completed her PhD from Bradford University, UK. She is Head of Pharmaceutics Department, Dubai Pharmacy College, UAE. She has teaching and research experience of about 25 years. She has published more than 44 papers in reputed journals and has been serving as an editorial board member of several pharmaceutical Journals. She is a member of various pharmaceutical associations. In addition to attending more than 40 training courses, conferences and seminars in Quality Assurance systems. Prof. Heyam 's expertise ranges from Preformulation, Product formulation, product testing and Stability, Quality Control, Assurance and GMP processes, validation in Pharmaceutical development of conventional dosage forms.

Every woman all over the world is obsessed to attain beauty an all ages in her life. However, the health implications that may result from the chronic use of toxic additives present in cosmetic products, urge the consumer to go for herbal cosmetic products. Therefore, the aim of this study to develop and evaluate the beneficial effects of rose hip oil (ROP) topical formulation in the suitable cream. Therefore, the aim of this study to develop and evaluate the beneficial effects of rose hip oil. “Rosa mosqueta” or “Rosehip”, is a generic name which covers about 70 different species of plants of the genus Rosa, as Rosa rubiginosa, Rosa moschata and Rosa canina. In the cosmetic field, the RHO has been used for decades due to transretinoic acid or natural tretinoin (between 0.01% and 0.1%). The emollient properties of the oil has been attributed to its high content of essential fatty and unsaturated acids, polyunsaturated fatty acid highlights: linoleic acid (54%), linolenic acid (17%) and oleic acid (16%). The method involves 1- preparation of Rose hip oil (ROP) (1%) in different different concentrations (0.5,1.0, and1.5)%. 2- The evaluations of all formulations (F1 to F3) were done on different parameters like pH, Viscosity, good consistency, homogeneity, appearance spreadibilty and stability. All formulations shows no redness, edema, inflammation and irritation during irritancy. However, formulation F3 was slightly better than the other formulations in the extent of photo protection, permeability and moisturizing abilities. Rose hip oil (ROP) in 1% formulations were efficient, stable and safe to use for skin as a multipurpose cosmetic product in skin hydration, sun protection, anti-aging and rejuvenating product. According to the findings this study is promising to further clinical studies.

Dima Almutrafi is working in department of Oral Medicine & Diagnostic Sciences, division of Oral medicine, Dental College, King Saud University, Saudi Arabia

Objectives: Many treatment modalities of Myofascial Pain exist; recent literature findings suggest the superiority of use of local anesthetics as a treatment of choice. The objective of this study is to compare the effectiveness of two of the most used local anesthetic agents: Lidocaine and Mepivacaine in the management of Myofascial Pain. Materials and Methods: A total of thirty patients, 20 females and 10 males, were assigned randomly. 50% of patients received Lidocaine and 50% received Mepivacaine. Trigger points injection in the orofacial region were given 4 time, 10 days between each injection, and 4 weeks follow up after the end of the treatment course. Pain levels were recorded on Visual Analogue Scale at the time of follow-ups, and half an hour after injection. Results: All groups, total of 30 patients, 20 females and 10 males (N=30), showed statistically significant improvements when comparing the pre and post treatment means. Both types of local anesthetics, Lidocaine and Mepivacaine, were equally effective for the management of Myofascial Pain, (p = 0.875). Mepivacaine treated group showed significantly less post injection tenderness compared to Lidocaine (p = 0.038). There was no relation between gender and treatment response. Both female and male patients reported similar response VAS scores (p = 0.818) Conclusion: No drug was superior to the other on the long term, thus the clinician choice is determined by drug availability and patient medical history.

Saleh mohammed Alamri completed his master degree of pharmaceutical biotechnology from De Montfort University of , UK in 2014. Mr. Alamri is working as a assistant director pharmacy of material management and part time researcher in Prince Sultan Military Medical City, KSA. He presented his work in national and international scientific conferences and meetings.

Pharmaceutical granules are very complicated heterogeneous systems consisting of discrete particulates having wide range of physical characteristics disseminated in the surrounded air voids. Granules and powders compression process in pharmaceutical industry consist of four to five consequent stages of phases. Recently, there are significant developments in numerous qualitative and quantitative experimental and numerical methods to probe the internal profile structure changes of powders and granular beds under certain external loads, but they are very expensive and required high technology. Simple colouring of pharmaceutical granules has been developed and adapted in this research project to investigate the macrobehaviour of freely flowing pharmaceutical granules (420-800μm) within confined die geometry. The granules fill to the die in multiple layers to visualise the changes in bed density. The results indicate that the die wall has limited influence of the early stage of the compression cycle and the granules layers compressed in homogenous manner. Increment of loading shows non- homogenous distribution of the coloured layers and the lower layer shows the minimal density changes. The interface of the layers shows considerable diffusion of the coloured granules.

Raman scattering is an inelastic phenomenon. Recent advances in electronics, lasers, optics, and nanotechnology have made Raman spectroscopy suitable to many areas of application. The present study highlights the application of Raman spectroscopy in drug analysis, where technique selection depends on various parameters, including ease of use and its ability to be reproducible and generate important results. The sample used to demonstrate the application of Raman mapping to characterise a pharmaceutical formulation in this study was a Quetiapine® tablet (150 mg); an atypical psychotropic. Raman microscopy was performed using a Thermo DXR™ 2 Raman Microscope. The laser wavelength was 532 nm and all experiments used a 900 lines/mm rotatable diffraction grating. With optimized measurement conditions for the investigation of spatial component distribution in solid dosage forms, two different objective sizes were compared to determine suitable measurement conditions for such pharmaceutical solid dosage forms. Raman maps were collected, effectively, by adjusting the mapping step size to increase the sampling area of each measurement. The Raman maps were generated using multivariate curve resolution to show the spatial distribution of the compounds within the formulation. All data pre-processing and analysis was performed using OMNIC™ version 9.

Mahmud Halima S completed her PhD in 2016 from Ahmadu Bello University Zaria, Nigeria. She is currently a Senior lecturer in same university. Her specialty is in evaluation and characterization of natural polymeric materials for use as excipient in both solid and liquid dosage forms. She has published more than10 papers in her area of specialty.Her passion is geared towards the development and standardization of raw materials for use in pharmaceutical industries. Presently her focus is on the use of many natural products available in her country, Nigeria as raw materials with different functionalities for industrial use to address importation problems.

The aim of this study was to investigate the ability of Adansonia digitata mucilage (ADM), a new hydrophilic plant polymeric material, in modifying Metoprolol tartarate (MPT) release from matrix tablet formulations in comparison with semi synthetic polymer-HPMC60SH4000 matrices. Modified release tablets of MPT were compressed via direct compression in the ratios of 1:1 and 1:4 drug polymer concentrations. The in vitro drug release in acid (pH 1.0) and Phosphate (pH 6.8) buffers, swelling and liquid uptake studies, drug release kinetics and mechanism were studied. Furthermore, in vivo studies were also carried out on 1:4 matrix tablets formulated with ADM.The matrix tablets produced were non friable and had acceptable weight uniformity. In vitro drug release of MPT was sustained for 8 h and 4 h in ADM and HPMC matrices of 1:1 drug/polymer ratio, respectively, whereas MPT release was controlled over 24 h for tablets with 1:4 drug/polymer ratio. The release profiles of all formulations showed pH independent release and no burst effect, even with high drug loading. The matrix integrity was maintained throughout in vitro dissolution with ADM matrix formulations, in contradiction to 1:1MPT/ HPMC matrix tablets which dissolved in 4h. This implied that the gel layer strength is higher in ADM matrix formulations. The drug release kinetics yielded a good fit in Higuchi model displaying a linear graphic which indicates controlled release in swellable polymers. The mechanism of MPT release was found to be anomalous fickian and Case II transport as a result of the swelling effects of the polymer.Statistical analysis confirmed that MPT release from ADM and HPMC60SH4000 (1:4 drug/polymer) was not significantly different (P > 0.05) . Besides, similarity factor (f2) showed that the in vitro release profiles of the sustained release (1:1) and controlled release formulations were similar in acid as well as phosphate buffer medium.. The in vivo bioavailability after oral administration of the test formulation (1:4 MPT/ADM) to dogs did not differ significantly from the reference marketed sustained release product.

The present paper describes the isolation, characterization, and antioxidant activity of a new coumarin; 5,8- dimethoxypsoralen which was isolated for the first time from the plant known as T. communis. The plant Tamus communis L. (Dioscoreaceae), commonly known as Black Bryony, is distributed all over the tropical and warm temperate regions of the world. The methanolic extract of the rhizomes of the plant was subjected to column chromatography over silica gel followed by preparative thin layer chromatography. Purification and structure determination have were operated by chemical analysis; mass spectroscopy (MS) and RMN H1 protons and C13.The antioxidant properties of 5,8- dimethoxypsoralen have been studied by two methods, evaluating its ability to protect RBCs from AAPH (2,2_-azobis (2-amidinopropane) hydrochloride) induced oxidative damage and lipid peroxidation. The inhibitory effect of lipid peroxidation showed that the inhibition of the compound (89.9 ± 0.1) are similar to that of BHT (86.03 ± 0.45) (p> 0.05) and showed significant anti-hemolytic activity expressed by a significant prolongation, the HT50 increased from 70.34 ± 2.15 min for control CTL (AAPH) to (130.485 ± 7.77 min). These results indicate that the roots of T.communis are a promising source of antioxidants.

Summary: The purpose of the study is to prevent the medicamentous iatrogenie and any use improper of the three psychotropic ones prescribed on blue ordinances with knowing (trihexyphenidyle.clonazepam.diazepam.lorazépam) Equipment and methods: Exploratory study carried out from1st September , 2015 to 30th July , 2016. An average of 200 ordinances per month was analyzed by applying circular N 250/96 relative to the psychotropic ones. Results: 10 falscified ordinances. 3 ordinances of which the duration of the treatment is higher than 3 months. 2 ordonnaces with overdose of the treatment. 3 without personal seal of the doctor. An ordinance with association against indiquee. Conclusion: The pharmaceutical analysis makes it possible to fight against the illicit traffic of psychotropic and to prevent the medicamentous iatrogenie.

Saida Touzouirtis working in department of Chemistry, University of Tizi-Ouzou, Algeria.

Emulsions play an important role in various fields such as in pharmaceuticals, cosmetics, food and drilling muds. In fact, the production and use of stable emulsions have been extensively examined in relation to the pharmaceutical and environmental applications, food industry, and petroleum production. Understanding, the required conditions for the formation of physically stable emulsions is an important area of research. Bentonite is considered as an environment friendly natural raw material and has already been used in several Pickering emulsions stability studies. It was included in the FDA Inactive Ingredients Database (oral capsules, tablets and suspensions, topical suspensions, controlled release transdermal films and vaginal suppositories) as well as in nonparenteral medicines licensed in the UK. Algerian bentonite is used for the first time as a Pickering emulsion stabilizing agent. In order to find out the emulsion optimal composition from the stability standpoint, a response surface methodology (RSM) was used with the help of Modde 6 software. For a better physical stability, methodology of experimental design is adopted with the Modde software. The purpose of the study was also to minimize the surfactant content in the formulation of pharmaceutical and cosmetic emulsions. Results show that 7% of bentonite and low concentration levels of cationic surfactant (CTAB, 0.02%) and salt (NaCl, 0.015M) provides a satisfactory physical stability after 22 months of ageing with emulsion mean particle size of the order of 20 µm. The rheological properties of the optimal formulation were investigated in order to assess its physical stability. Viscoelasticity and flow curves show that G' modulus is greater than G”, which indicates that the emulsion behaves like a viscoelastic fluid, with a rigid structure showing a high viscosity (500Pa.s) at the zero state and a low viscosity (0.05Pa.s) at the infinite state.

Soghra Khabnadideh, Pharm D. Doctor (Ph.D.- Medicinal Chemistry) now is a professor of Medicinal Chemistry in Faculty of Pharmacy, Shiraz University of Medical Sciences. She has completed her Ph.D at the age of 34 years from Isfahan University of Medical Sciences and postdoctoral studies from, School of Pharmacy, Cardiff University. She is the head of Department of Medicinal Chemistry, Shiraz University of Medical Sciences. She is a member of Iranian Association of Pharmaceutical Scientists and also Pharmaceutical Science Research Center, Shiraz University of Medical Sciences. She has published more than 35 papers in reputed journals. Her research field is synthesis of heterocyclic compounds in the area of antifungal, antibacterial and anticancer agents. She had a 6 month fellowship to University of New Sought Wales (UNSW), Sydney and worked on synthesis in ionic liquids.

A simple and efficient method has been developed for synthesis of some benzoxazole derivatives. In the first step, different 2-aminophenol analogues were reacted with various aldehydes in the presence of titanium supported nano-silica to produce an imine intermediate. Then the imine was oxidized to get the final benzoxazole compounds. Eleven derivatives were synthesized (c-m) via this simple and environmentally friendly procedure with high yields. The identification and characterization of all the synthesized compounds were carried out by elemental analysis, melting point, thin layer chromatography, FT-IR and 1H NMR spectral data. Simple methodology (only 2 steps) with high yields for the reactions and easy work-up are the advantages of our method. All the compounds were screened for antibacterial and antifungal activity by broth micro dilution methods as recommended by CLSI. Of the tested compounds 2-(2,4-Dichlorophenyl)-1,3-benzoxazole (g), and 2-(4-Chlorophenyl)-1,3-benzoxazole (f) inhibited the growth of all examined fungi and also 2-(4-Nitrophenyl)-1,3-benzoxazole (c) exhibited inhibitory activities against the tested yeasts. Finally in order to compare the synthesized compounds based on theirs structures and activities, SAS (Structure Activity Similarity) map analysis was also performed.

Taghrid Shehteh Abu Hassan has completed her Bachelor degree of pharmaceutical Science at Philadelphia University, Jerash - Jordan in 2003 and completed her Master degree of clinical pharmacy at Queen's University, Belfast UK in 2014. She has experience in hematology/oncology hospital pharmacy, pharmacy management, aseptic pharmaceutical preparation, inpatient, and ambulatory setting. Currently she provides care as the senior pharmacist for unit dose/Inpatient pharmacy unit at National center for cancer care and research Qatar. She is a preceptor for Qatar University students. She has experience in peer-reviewed publications and poster presentations.

Medication Reconciliation (Med.Rec) is a major intervention which reduces medication discrepancies and subsequent patient harm at different patients’ care transitions. Data on the incidence of the unintended medications discrepancies ranges from 40–50% upon admission to acute care hospitals and 40% at hospitals discharges. Outpatient pharmacist play a lead role in detecting medication related problems; this urges the need to translate their qualitative values into quantitative measures. Primary objective to investigate the impact of outpatient pharmacists’ interventions during discharge reconciliation, in reducing medication errors and discrepancies. Secondary objective to detect the most common medication related problems. A prospective observational study, conducted at a 62-bed tertiary care (National Centre for Cancer Care & Research) in Qatar. All discharged patients were included in the study over duration of 10 months. Patients who were discharged from the chemotherapy infusion unit were excluded. A standardized intervention form was generated to document interventions. Collected data were categorized into medication error or medication discrepancy. A statistical analysis included exploratory analysis and descriptive statistics using STATISTICA 11.0 Version. Total of 591 discharge prescriptions included, 278 (47%) required pharmacist interventions; with 190 medication discrepancies and 122 medication errors. The most common medication related problems were incomplete orders (34%) and prescribing restricted medication without privilege (29%). Outpatient pharmacists have a significant role towards detecting and reducing medication errors and discrepancies upon patient discharge. However, despite their effective interventions, most of these medications related problems are preventable. An improved quality process and awareness can create an efficient medication safety environment.