Jhon Raphael M Jimenez
Our Lady of Fatima University, Philippines
Title: Determination of the antifungal property of the semi-purified glycosides from the extract of Mollugo oppositifolia Linn. (Sarsalida) versus Aspergillus niger and Candida albicans
Biography
Biography: Jhon Raphael M Jimenez
Abstract
The prevalence of resistance to antifungal agents significantly increased in the past decade. So it led to the discovery of new classes of antifungal compounds to that fungal infection. The plant Mollugo oppositifolia (Molluginaceae) which is locally known as “Malagoso or Papait” have been reported in favor of various traditional uses. Mollugo oppositifolia is reported to possess antifungal property due to presence of glycosides. This led to the result of discerning Sarsalida as an antifungal agent. Methanolic extract of Mollugo oppositifolia was seen the presence of steroid glycosides, flavonoids, tannins, glycosides and carbohydrates according to phytochemical screening. Stas-Otto Method was used for the semi-purification of glycosides. The semi-purified extract of Mollugo oppositifolia underwent Physicochemical Analysis. Test organisms undergone observations were Aspergillus niger and Candida albicans. Antifungal Susceptibility Testing was used to carry out and two-fold serial tube dilution method was used to determine the Minimum Inhibitory Concentration. Aspergillus niger exhibited average significant inhibition of 13.67 mm (±1.6704) and Candida albicans exhibited average significant inhibition of 18.67 mm (±1.6704), both of which are statistically significant.