Pardeep K. Gupta
University of the Sciences in Philadelphia, USA
Title: Design of Growth-Hormone Binding Peptide and its use as a Vehicle for Nanoparticle-Based Delivery of Growth-Hormone
Biography
Biography: Pardeep K. Gupta
Abstract
A novel peptide that mimics the binding properties of growth hormone binding protein (GHBP) was designed and synthesized. This peptide, termed growth hormone binding peptide (GHBpep) contains the functional epitopes of GHBP that allow it to bind to GH. This peptide was studied for its binding to GH in solution to characterize its binding affinity and thermodynamics of the binding process. These studies show that GHBpep binds to GH in a 1:1 ratio. GHBpep was covalently linked to bio-functionalized biodegradable PLGA nanoparticles. Reversible binding of GH to immobilized GHBpep was studied using equilibrium binding studies. The results of these studies indicate that GH can be loaded onto functionalized biodegradable nanoparticles in a reversible manner, and can be used as a delivery system for GH.