12^th Annual Pharma Middle East Congress

Biography

Biography: Sahar S Abd El-Rahman

Abstract

Introduction: AflatoxinB1 (AFB1) is well-known as a feed borne-immunosuppressive mycotoxin. Nanotechnology is a powerful new technology for reconstructing nature at the atomic and molecular level. This study was conducted to determine the efficacy of nano-composite magnesium oxide and silicon oxide (MgO-SiO₂) to reduce the toxic effects of AFB1on the immunity of adult male rats for 8 weeks. Experimental design: Animals were divided into a control (Gp1) and three experimental groups (Gps); Gp2 received feed contained 0.5g/kg nano-composite MgO-SiO₂, Gp3 received feed contained 200ppb/kg AFB1. While rats of Gp4 received feed contained 200ppbAFB1/kg and 0.5g/kg the nano-composite. Methods: Cellular and humoral immune responses, as well as histopathological examination and caspase-3 expression in liver, spleen, and GIT were all evaluated. Residual concentration of AFB1was determined in serum, liver and fecal samples. The obtained data were statistically analyzed. Results: AFB1markedly reduced body weight gain and food and water consumption. Both cellular immune response (total and differential leukocytic count, neutrophils’ phagocytic activity, lymphocyte transformation, macrophage activity and serum lysozyme activity) and humoral immune response (serum total protein and its fractions as estimated by SDS- PAGE electrophoresis) were severely reduced by AFB1. Moreover, AFB1induced marked histological alterations and apoptosis in liver, spleen, and GIT. The addition of nano-composite MgO-SiO₂ to feed containing AFB1 could markedly alleviated the deleterious effects of AFB1. Conclusion: These findings suggested that nano-composite MgO-SiO₂ has high affinity to adsorb AFB1and can effectively modulate its toxicity in rats. Impact statement: Nano-composite MgO-SiO₂ may offer a novel approach for the preventive management of aflatoxicosis in animals.