Sahar M Kamal has received her MD in Basic and Clinical Pharmacology from Pharmacology Department, Faculty of Medicine, Ain Shams University during the period of May 1997- May 2001. Currently, she is working as Professor of Pharmacology in Ain Shams University. She lectures on Basic & Clinical Pharmacology in both faculties of Medicine and Dentistry in Ain Shams University, Cairo, Egypt. She is serving as an editorial member of several reputed journals like Journal of Neurological Disorders and expert Reviewer for Journal of Experimental Pharmacology. She has authored 24 research articles.
The frequent coexistence of depression in epileptic patients raises the issue of simultaneous use of antidepressants along with antiepileptic drugs in the management of such cases. However, it is necessary to evaluate the safety of these antiepileptic/antidepressant drug combinations. The present study investigates the effect of the antidepressant paroxetine (a selective serotonin reuptake inhibitor) administered alone or in combination with the anti-epileptic drug sodium valproate on chemo-convulsions induced by Picrotoxin (PTX). Seizure score was recorded in vivo, and the levels of thio-barbituric acid-reactive substances and gamma amino-butyric acid (GABA) were measured in the nucleus accumbens of the tested groups of mice. The results show enhancement of seizure severity with significant reduction in GABA levels upon PTX treatment that were reversed by its combination with sodium valproate. On the other hand, paroxetine administered in combination with sodium valproate provided significant protection against PTX-induced convulsions as well as a significant increase in GABA levels in selected brain areas. These results favor their application in management of epilepsy-depression comorbidities.
After finishing his undergraduate study of pharmaceutical Sciences with the grade of honour, Dr Mohamed has been awarded his PhD in Infection, Immunity and Inflammation from Leicester University, UK. He served as a lecturer of Microbiology and Immunology, Al-Azhar University, Cairo, Egypt, and he is currently an assistant professor of Microbiology and Immunology, University of Dammam, KSA. Dr Mohamed has published a group of research papers in immunology and tumour pharmaco- and immunotherapy in high impact journals, and he volunteer as a reviewer/ associate editor in a number of internationally reputed journals of the field. In addition, he is a member in many professional societies, such as; American Association of Cancer Research, Saudi Society of Infectious Diseases and Microbiology, European Association of Cancer Research, British Society of Immunology, Egyptian Pharmacy Syndicate. His research interests include; the malignant diseases management via cellular immunotherapy and cancer vaccines and the antimicrobial resistance patterns of Mtb.
Introduction: Due to side effects and resistance to traditional chemotherapeutic agents, new therapeutic strategies are requested for hematologic tumour management. Adoptive T cell immunotherapy represents an alternative effective, long-acting approach of minimal side effects. This study evaluates the ability of hybrid cells, generated by fusion of leukaemia tumour cells with Antigen Presenting Cells (APC) to stimulate tumour-specific cytotoxic T cells with the ability to recognise and destroy parent tumour cells.\r\n\r\nMethods: Acute myeloid leukaemia tumour cells were fused to APC and the growth of the hybrid cells were chemically selected. The hybrid cell growth was maintained in tissue culture conditions, and subjected to phenotypic evaluation using flow cytometer. Tumour antigen expression was detected by RNA extraction followed by reverse transcription PCR using antigen specific primers. Subsequently, hybrid cells were allowed to induce T cell specific response from PBMC of normal donors. Cytotoxicity and IFN-γ ELISpot assays were performed to evaluate the functional activity of the activated T cells.\r\n\r\nResults: The hybrid cells expressed relevant tumour antigens in context of MHC I/II, in addition to T cell co-stimulatory molecules. The immortalised hybrids induced allogeneic cellular T cell responses of both cytotoxic CD8 and CD4 types. More importantly, the induced cytotoxic CD8+ T cells successfully responded by IFN-γ release following co-culture with the target tumour cells and induce target cell apoptosis in cytotoxicity assays.\r\n\r\nConclusion: Hybrid cells of this type had the potential to induce specific T cell response and could be a successful immuno-therapeutic, T cytotoxic cell inducer to be used for adoptive cellular management of hematologic cancers. \r\n